A high-throughput BIA-MPA method for the simultaneous determination of amiloride and furosemide
| Autor: | W. P. da Silva, Rodrigo A.A. Munoz, Mariana C. Marra, Polyana Fernandes Pereira, Eduardo M. Richter |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Detection limit
Chromatography Chemistry General Chemical Engineering 010401 analytical chemistry General Engineering Analytical chemistry Furosemide 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences High-performance liquid chromatography Amperometry 0104 chemical sciences Analytical Chemistry Amiloride Dilution Electrode medicine 0210 nano-technology Throughput (business) medicine.drug |
| Zdroj: | Analytical Methods. 8:7959-7965 |
| ISSN: | 1759-9679 1759-9660 |
| DOI: | 10.1039/c6ay02506d |
| Popis: | In this work, a simple and fast batch injection analysis system with multiple pulse amperometric detection (BIA-MPA) was developed for the simultaneous determination of amiloride (AMD) and furosemide (FMD). A sample plug (150 μL) was directly injected into the unmodified boron-doped diamond (BDD) electrode immersed in 0.1 mol L−1 borate buffer (pH = 10). Simple and fast sample pre-treatment steps were required (dissolution and dilution). The analytical characteristics of the proposed method include good stability (RSD < 1.1%; n = 20), low detection limits (0.13 and 0.94 mg L−1 for AMD and FMD, respectively), high throughput (72 injections per h) and minimal waste production (∼150 μL by analysis). The results obtained with the BIA-MPA method were compared to those obtained by HPLC; similar results were obtained (at a 95% confidence level). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|