Rescue In Vitro Fertilization Method for Legacy Stock of Frozen Mouse Sperm

Autor:	Tomoko Umeno, T. Kondo, Kiyoko Fukumoto, Yuko Nakamuta, Toru Takeo, Yukie Haruguchi, Shuuji Tsuchiyama, Yumi Takeshita, Naomi Nakagata
Rok vydání:	2014
Předmět:	endocrine system In vitro fertilisation urogenital system medicine.medical_treatment Sperm cryopreservation Sperm washing Biology Insemination Sperm Cryopreservation Andrology Human fertilization Immunology medicine Animal Science and Zoology reproductive and urinary physiology
Zdroj:	Journal of Reproduction and Development. 60:168-171
ISSN:	1348-4400 0916-8818
DOI:	10.1262/jrd.2013-141
Popis:	Sperm cryopreservation has been widely adopted for maintenance of the genetically engineered mouse (GEM). The cryopreserved sperm are being exchanged among many institutes worldwide. However, the recipients are not always able to obtain high fertilization rates with the frozen sperm shipped from senders. In this study, we cryopreserved mouse sperm via various methods and performed in vitro fertilization (IVF) in which the combination of methyl-beta-cyclodextrin for sperm preincubation and reduced glutathione for insemination was used (the MBCD-GSH IVF). In addition, frozen sperm sent from the Jackson Laboratory (USA) were thawed and used for IVF in the same manner. The fertilization rates of both the sperm cryopreserved via the methods applied in some countries and the cryopreserved GEM sperm improved when used with the MBCD-GSH IVF method. Therefore, we strongly believe that the MBCD-GSH IVF method brings about relatively high fertilization rates with any strain of frozen mouse sperm.
Databáze:	OpenAIRE
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