Analysis of Genetic Diversity in Eleven Tomato (Lycopersicon esculentum Mill.) Varieties using RAPD Markers

Autor:	Sujay Kumar Bhajan, Sonia Khan Sony, Nadra Tabassum, Mohammad Nurul Islam
Rok vydání:	2013
Předmět:	Genetic diversity Dendrogram Plant Science Biology biology.organism_classification Lycopersicon RAPD Horticulture Genetic distance Genetic marker Botany Genetic variation Cultivar Biotechnology
Zdroj:	Plant Tissue Culture and Biotechnology. 23
ISSN:	1818-8745 1817-3721
DOI:	10.3329/ptcb.v23i1.15559
Popis:	Genetic diversity of 11 tomato ( Lycopersicon esculentum Mill.) varieties was assessed through RAPD analysis. Twenty arbitrary oligonucleotide primers used in the RAPD ‐ PCR produced a total of 584 different marker bands with an average of 29.2 bands per primer. Based on the banding pattern 94.168% polymorphism observed among the tomato varieties. Size range of amplified DNA bands varied from 0 . 1 ‐ 10 k b. A total of 15 unique bands were amplified from the genome of the 11 tomato varieties. The values of pair ‐ wise genetic distances ranged from 0.1838 - 0.9049, indicating the presence of wide genetic diversity. The dendogram constructed based on phylogenetic relationship analysis revealed that the highest genetic diversity (0.9049) found between the variety BARI Tomato 8 and RATAN whereas the lowest (0.1838) between the variety BARI Tomato 9 and MADHURI. The dendogram has segregated the 11 tomato varieties into two major clusters. RATAN and SPECIAL ‐ OP formed cluster 1 and rest 9 varieties viz ., ROMA ‐ VF, MARGLOBE, MADHURY and BARI Tomato 9, 2, 6, 14, 8 and 3 have constituted the second cluster. Plant Tissue Cult. & Biotech. 23 (1): 49 ‐ 57, 2013 (June) DOI: http://dx.doi.org/10.3329/ptcb.v23i1.15559
Databáze:	OpenAIRE
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