Detection of Immunoglobulin M and Immunoglobulin G Antibodies Against Orientia tsutsugamushi for Scrub Typhus Diagnosis and Serosurvey in Endemic Regions
| Autor: | Suchit Kamble, Mohan D Gupte, Manish M Gupte, Arati Mane, Deepak Gadkari, Suvarna Sane, Manoj V Murhekar, Vijay P. Bondre, Jagadish M. Deshpande |
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| Rok vydání: | 2020 |
| Předmět: |
Orientia tsutsugamushi
biology business.industry Outbreak Scrub typhus bacterial infections and mycoses biology.organism_classification medicine.disease Virology Immunoglobulin G Serology 03 medical and health sciences 0302 clinical medicine Immunoglobulin M 030225 pediatrics Clinical diagnosis Pediatrics Perinatology and Child Health biology.protein medicine 030212 general & internal medicine Antibody business |
| Zdroj: | Indian Pediatrics. 57:1131-1134 |
| ISSN: | 0974-7559 0019-6061 |
| Popis: | To estimate the regional cutoff of optical density (OD) values for immunoglobulin M (IgM) antibodies against Orientia tsutsugamushi in serum and cerebrospinal fluid (CSF) for clinical diagnosis of scrub typhus and immunoglobulin G (IgG) antibodies in serum for sero-epidemiology in Gorakhpur, Uttar Pradesh, India. We used data from a serological investigation of acute encephalitis syndrome patients (n=407) during the 2016 outbreak in Gorakhpur, India to determine the cutoff for OD values for IgM antibodies, and from community-based serosurveys (n=1991) to estimate the cutoff for OD values for IgG antibodies. We determined regionally relevant cutoff for OD values of 0.76 for IgM antibodies in serum and 0.22 in cerebrospinal fluid for scrub typhus diagnosis. For serosurveys, IgG antibody cutoff was 1.5. We have proposed locally relevant cutoffs for scrub typhus endemic regions, which may be useful for correctly classifying infected population. |
| Databáze: | OpenAIRE |
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