| Autor: |
Ian Dobbie, Erika Tse-Luen Wee, Laure Plantard, Xiang Hao, Claire Mitchell, Glyn Nelson, Judith Lacoste, Nadia Halidi, Alexander Corbett, Hella Hartmann, Yury Belyaev, Stanley Schwartz, Penghuan LIU, Rainer Heintzmann, Ioannis Alexopoulos, SANTOSH PODDER, Roland Nitschke, Laurent Gelman, Aurelien Dauphin, Alexander L. Payne-Dwyer |
| Rok vydání: |
2022 |
| Popis: |
This protocol focuses on measuring the microscope's lateral and axial resolution, essential for reporting size measurements of near-resolution limit objects or distances between them. Here resolution is expressed as the Full Width at Half Maximum (FWHM) of a measured Point Spread Function (PSF) of sub-resolution size beads. PSF is highly related to objective quality and condition, but also depends strongly upon other parameters ranging from sample preparation to signal detection. Monitoring PSF over time will identify possible aberrations in the system (e.g., damaged, unclean objective, defective or not adapted oil, etc.). We define test sample preparation, image acquisition, and data analysis protocols for point scanning and spinning disk confocal microscopes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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