Phenolic compounds inhibit cellulase and xylanase activities of Cellulomonas flavigena PR-22 during saccharification of sugarcane bagasse
| Autor: | Francisco Javier Ríos-Fránquez, Ana C. Ramos-Valdivia, Teresa Ponce-Noyola, Héctor M. Poggi-Varaldo, Juan Carlos Santana-Morales, Enrique González-Bautista, Eliseo Cristiani-Urbina |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine General Chemical Engineering Energy Engineering and Power Technology Lignocellulosic biomass macromolecular substances Cellulase 01 natural sciences 03 medical and health sciences Hydrolysis chemistry.chemical_compound 010608 biotechnology Enzymatic hydrolysis PEG ratio Lignin Food science biology Chemistry Organic Chemistry technology industry and agriculture food and beverages 030104 developmental biology Fuel Technology biology.protein Xylanase Bagasse |
| Zdroj: | Fuel. 196:32-35 |
| ISSN: | 0016-2361 |
| Popis: | The enzymatic hydrolysis of sugar cane bagasse (SCB) releases simple fermentable sugars however, phenolic compounds (PCs) are also released from the lignin present in the structure of the lignocellulosic biomass limiting the production of sugars and therefore the fermentative process to convert these sugars to bioethanol. During the saccharification of SCB with holocellulases from Cellulomonas flavigena PR-22, 46 μg mL −1 of PCs were produced, which inhibited 87 and 74% carboxymethyl cellulase (CMCase) and xylanases respectively resulting in a low concentration of reducing sugars (5.5 g L −1 ) at the end of the process. Polymers such as polyethylene glycol 4000 (PEG) and polyvinylpyrrolidone (PVP) can prevent this inhibition due to their ability to chemically adsorb PCs. PEG and PVP were added to C. flavigena PR-22 enzyme extracts at 0.4% (w/v). PEG 4000 prevented CMCase inhibition, maintaining the original activity after the saccharification process, whereas xylanase production was increased by 47% when PVP was added. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect