Enzymatic hydrolysis of microalgal cellulose for bioethanol production, modeling and sensitivity analysis
| Autor: | Mehdi Zamani, Hanieh Shokrkar, Sirous Ebrahimi |
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| Rok vydání: | 2018 |
| Předmět: |
Chromatography
biology 020209 energy General Chemical Engineering Organic Chemistry Energy Engineering and Power Technology Biomass 02 engineering and technology Cellulase Cellobiose 010501 environmental sciences 01 natural sciences chemistry.chemical_compound Fuel Technology chemistry Product inhibition Enzymatic hydrolysis Yield (chemistry) 0202 electrical engineering electronic engineering information engineering biology.protein Fermentation Cellulose 0105 earth and related environmental sciences |
| Zdroj: | Fuel. 228:30-38 |
| ISSN: | 0016-2361 |
| Popis: | A kinetic model was developed to describe the enzymatic hydrolysis of microalgal cellulose. In the kinetic model, the factors including product inhibition, temperature, and pH were considered. This model combined two reactions for hydrolyzing algal cellulose to cellobiose and glucose and one reaction for cellobiose breakdown to glucose. Results showed that the highest glucose yield (57%) was achieved at microalgal biomass concentration of 50 g/L, pH 5, and temperature of 50 °C. Moreover, the sensitivity analysis was carried out on each kinetic model parameter. This analysis indicated that k 3 ′ and km3 in reaction R3 (cellobiose to glucose) are the most influential parameters during enzymatic hydrolysis of algal cellulose. Finally, the microalgal biomass loading experiment demonstrated that cellulase could be used thrice without compromising on the glucose yield. Fermentation of concentrated sugar medium with Saccharomyces cerevisiae produced ethanol (12.87 g/L) with yield (0.46 g ethanol/g glucose). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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