Effects of Multipotent Mesenchymal Stromal Cells of the Human Umbilical Cord and Their Combination with Melatonin in Adult and Aging Mice with a Toxic Cuprizone Model of Demyelination

Autor: O. K. Toporova, N. A. Utko, I. F. Labunets
Rok vydání: 2021
Předmět:
Zdroj: Advances in Gerontology. 11:173-180
ISSN: 2079-0589
2079-0570
Popis: The content of malondialdehyde and the activity of antioxidant enzymes in the brain, as well as the behavior parameters (open field test and rotarod test), were evaluated in 6–7- and 15–17-month-old mice with a cuprizone demyelination model. They were injected with human umbilical-cord multipotent mesenchymal stromal cells (MMSCs), including a combination with the hormone melatonin. The neurotoxin cuprizone was administered daily with food for 3 weeks, and MMSCs were administered on the tenth day of the cuprizone diet (0.5 × 106 cells, once intravenously); melatonin was administered on days 11–21 of this diet (1 mg/kg, at 18:00, daily). It was found that cuprizone increased the malondialdehyde content and decreased the activity of antioxidant enzymes in the brains of mice of both age groups; the locomotor, emotional, orientational-exploratory activity and the muscle tone also decreased. The transplantation of MMSCs led to an increase in the number of crossed squares and grooming activities in adult mice and in the rearing activity and rotarod retention time in the older ones. The injection of cells in combination with melatonin enhanced their effect on the behavior parameters in mice of both age groups and, in addition, increased the number of boluses and rotarod retention time in adult mice and the number of crossed squares and grooming activity in aging animals. After MMSCs transplantation, there is a decrease in the malondialdehyde content in the brains of adult mice; the effect persists when combined with melatonin. The activity of glutathione peroxidase and glutathione reductase increases in the brain of mice of different ages injected with cells and melatonin. Thus, the manifestations of the positive effect of transplanted MMSCs on the behavior of mice with a cuprizone model of demyelination depend on their age. The combination of cells with melatonin leads to an increase in positive changes in the behavior parameters in mice of different ages. This may be associated with the more pronounced antioxidant effect in the brain.
Databáze: OpenAIRE
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