Effect of Freeze-Thawing Process on the Size and Lamellarity of PEG-Lipid Liposomes
| Autor: | Masaharu Ueno, Supaporn Sriwongsitanont |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Phosphatidylethanolamine
Liposome Morphology (linguistics) Chromatography technology industry and agriculture Multilamellar vesicles Freeze thawing law.invention chemistry.chemical_compound chemistry law Electron micrographs Peg lipid Biophysics lipids (amino acids peptides and proteins) Electron microscope |
| Zdroj: | The Open Colloid Science Journal. 4:1-8 |
| ISSN: | 1876-5300 |
| DOI: | 10.2174/1876530001104010001 |
| Popis: | The influence of the freeze-thawing process on the size and lamellarity of multilamellar PEG-lipid liposomes prepared from a mixture of egg yolk phosphatidilcholine (EggPC) and distearoyl phosphatidylethanolamine polyethyle- neglycol (DSPE-PEG) 2000 was investigated. Trapped volume measurement, quasielastic light scattering (QELS) and freeze-fracture electron microscopy were used to estimate the morphology and lamellarity of liposomes. During the freeze-thawing process, the lamellarity of multilamellar vesicles (MLVs) depended strongly on both PEG-lipid concentra- tion and the number of freeze-thaw cycles. The decrease in the number of lamellae was a function of the number of freeze-thaw cycles. The increase in trapped volume coincided with the decrease in the number of lamellae observed in electron micrographs. After comparing the results obtained from EggPC/DSPE-PEG2000 MLV and from pure EggPC MLV, it was concluded that during the freeze-thawing the liposomes with DSPE-PEG2000 achieved a unilamellar structure more readily than the pure EggPC liposomes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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