| Popis: |
Gene biochip imaging and scanning technology is a more advanced gene diagnosis technology after fluorescence quantitative PCR technology, second-generation sequencing technology and digital PCR technology. Due to the great influence of fluorescence microscopy system, the imaging and scanning technology of gene biochip with high imaging efficiency and accuracy has become a hot topic widely concerned by scholars. In this paper, two-channel laser was used as the excitation light source of the biochip, and the fluorescence imaging system of the switchable fluorescence filter was designed to realize the detection of FAM and HEX biological fluorescence signals; According to the working principle of the gene biochip imager, the finite element analysis method is used to analyze the thermal structure of the optical system, and the internal structural parameters of the optical system are optimized according to the simulation results. According to the optimization result, the illumination time of each object pixel is controlled to utilize the fluorescence information more efficiently, and the fluorescent bleaching effect is reduced without sacrificing the image quality, and the PCR amplification oil droplet quantitative reading and display is performed in real time. The gene biochip imaging system proposed in this paper can be used to image, obtain, read, process and display the detected chip with a resolution of 10 m/ pixel, a minimum detection limit of < 10 fluorescent molecules/square micron, and a sample detection repeatability of < 10%.The results show that the gene biological chip imaging system designed by this research has the advantages of low noise, low cost and fast scanning speed, and provides a theoretical basis for precision medical fields such as early diagnosis and cure evaluation of clinical tumors. The results show that the gene biochip imaging system designed by this research has the advantages of low noise, low cost and fast scanning speed, and provides a theoretical basis for precision medical fields such as early diagnosis and cure evaluation of clinical tumors. |
