Targeted transcript analysis revealed association of suboptimal expression of certain endometrial immunity-related genes with disparate uterine diseases in zebu cows

Autor: S. Mallick, Satparkash Singh, Ashok Kumar Mohanty, S. Kumar, B.R. Maharana, R. K. Baithalu, Tushar Kumar Mohanty, Arumugam Kumaresan
Rok vydání: 2019
Předmět:
Zdroj: Tropical Animal Health and Production. 51:2493-2503
ISSN: 1573-7438
0049-4747
Popis: The onset of uterine infection during postpartum period compromises uterine health, fertility, and productivity of dairy cattle. Endometrial innate immunity plays a key role in eliminating uterine infection and keeping the uterus healthy. Hence, the present study has been designed with the hypothesis that altered endometrial immune response around calving may compromise uterine health during postpartum period. Expression of interleukins (IL-1β, IL-6, IL-8, IL-10, and TNF-α), prostaglandin synthase (PGFS, PGES), and antimicrobial peptides (beta-defensins (BDEF-4, BDEF-5), lingual antimicrobial peptide (LAP), and calcium-binding proteins (S100A8, S100A9, and S100A12) in endometrial tissues on the day of calving was studied using qRT-PCR, and circulating concentrations of prostaglandin E and F metabolites (PGEM and PGFM) during peripartum period (on days − 7, − 4, − 1 (before calving), 0 (on the day of calving), + 1, + 4, and + 7 (post calving)) of normal (healthy) cows (n = 11) that did not develop postpartum uterine infection and cows that developed puerperal metritis (n = 7) and clinical endometritis (n = 6) were studied. Endometrial expression of IL-1β, TNF-α, BDEF-4, BDEF-5, S100A8, S100A12, and PGFS was higher (P
Databáze: OpenAIRE
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