CНИЖЕНИЕ ЭКСПРЕССИИ ГЕНА NR0B2 ПРИ СВЕТЛОКЛЕТОЧНОМ РАКЕ ПОЧКИ СВЯЗАНО С ГИПЕРМЕТИЛИРОВАНИЕМ ЕГО ПРОМОТОРНОЙ ОБЛАСТИ, 'Молекулярная биология'
| Autor: | Elena A. Pudova, A V Snezhkina, Maria S. Fedorova, Anna V. Kudryavtseva, Kirill M. Nyushko, Boris Alekseev, Zulfiya G Guvatova, Asiya F. Sadritdinova, Maria V Savvateeva, Dmitriy A. Shagin, Andrew R. Zaretsky, Alexey A. Dmitriev |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Tumor suppressor gene NR0B2 Gene Bisulfite sequencing Promoter General Medicine Biology medicine.disease 03 medical and health sciences Clear cell renal cell carcinoma 030104 developmental biology Downregulation and upregulation DNA methylation Cancer research medicine Transcriptional regulation |
| Zdroj: | Молекулярная биология. :482-488 |
| ISSN: | 0026-8984 |
| Popis: | Clear cell renal cell carcinoma (ccRCC) is a common urologic malignancy. Understanding of the transcriptional regulation of oncogenes and tumor suppressor genes involved is critical for the development of the treatments for renal tumors. Using ccRCC subdivision of the TCGA dataset, we identified NR0B2 encoding orphan nuclear receptor as a tumor suppressor candidate in renal tissue. In independent cohort of primary renal tumors, quantitative PCR experiments confirmed significant suppression of NR0B2 mRNA in 86% of ccRCC samples studied. In 80% of these cases, we detected the hypermethylation of the NR0B2 pro-moter region. These results suggest that NR0B2 is a tumor suppressor gene in ccRCC, and that the hypermethylation of promoter region is the main mechanism of its downregulation. |
| Databáze: | OpenAIRE |
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