Characterization of the Action of Antibiotics and Essential Oils against Bacteria by Surface-Enhanced Raman Spectroscopy and Scanning Electron Microscopy
| Autor: | Tiberiu Szöke-Nagy, Nicoleta Elena Dina, Alin Sebastian Porav, Cristian Coman, Carmen Tripon, Bogdan Ionut Cozar |
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| Rok vydání: | 2018 |
| Předmět: |
Clinical Biochemistry
02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry symbols.namesake Electrochemistry medicine Denaturation (biochemistry) Spectroscopy chemistry.chemical_classification biology Chemistry 010401 analytical chemistry Biochemistry (medical) Glycosidic bond Surface-enhanced Raman spectroscopy 021001 nanoscience & nanotechnology biology.organism_classification Enterococcus durans 0104 chemical sciences Mechanism of action symbols Biophysics Aeromonas media medicine.symptom 0210 nano-technology Raman spectroscopy Bacteria |
| Zdroj: | Analytical Letters. 52:190-200 |
| ISSN: | 1532-236X 0003-2719 |
| Popis: | The description of the main structural alterations generated by the interaction between pathogenic microorganisms and antibiotic agents, either standard antibiotics or volatile plant derived oils, is an essential topic in understanding drug action mechanisms at the molecular level. To investigate this effect, an original approach based on ultrasensitive vibrational surface-enhanced Raman spectroscopy (SERS) and scanning electron microscopy (SEM) was used. Single-bacteria SERS spectra, recorded in the 600–1800 cm−1 wavenumber interval are reported for two bacterial strains: Enterococcus durans and Aeromonas media. Spectra-structure correlations for all types of localized vibrations, including bands assigned to proteins, saturated lipids, glycosidic linkages, and nitrogenous bases, are also discussed. SEM images revealed that the mechanism of action of antibiotic agents against the pathogenic microorganisms involves a complete denaturation of the bacterial membrane, which induces bacterial cells apo... |
| Databáze: | OpenAIRE |
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