In vitro regeneration and Agrobacterium-mediated transformation of male- sterile marigold (Tagetes erecta L.)
| Autor: | Yasushi Murai, Mikiko Uesugi, Shinya Kajita, Yoshihiro Katayama, Mana Narushima, Nuoendagula, Yosuke Iimura |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Molecular breeding biology Agrobacterium fungi Plant Science Genetically modified crops Beta-glucuronidase biology.organism_classification 01 natural sciences 03 medical and health sciences Transformation (genetics) 030104 developmental biology Tagetes Shoot Botany Agronomy and Crop Science 010606 plant biology & botany Biotechnology Explant culture |
| Zdroj: | Plant Biotechnology. 34:125-129 |
| ISSN: | 1347-6114 1342-4580 |
| DOI: | 10.5511/plantbiotechnology.17.0530a |
| Popis: | In this study, we devised a method for the in vitro regeneration and subsequent genetic transformation of male sterile marigold. To our knowledge, this is the first report of generation of transgenic plants with a single genotype of marigold via Agrobacterium-mediated transformation. We obtained four transgenic lines from two independent experiments with 496 leaf explants, which were inoculated by an Agrobacterium strain LBA4404 harboring the plasmid, pIG121-Hm. Although the efficiency of the transformation in our system was low, stable expression of uidA gene in adventitious shoots and compound leaves could be detected in β-glucuronidase histochemical analysis. This protocol contributes to the progress of genetic studies and molecular breeding of this species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|