Inulin fructotransferase (DFA III-producing) from Leifsonia sp. T88-4

Autor:	Ken'ichi Ohtsubo, Mitsuru Yoshida, Kazutomo Haraguchi
Rok vydání:	2006
Předmět:	chemistry.chemical_classification Chromatography Polymers and Plastics Molecular mass biology Chemistry Dimer Organic Chemistry Size-exclusion chromatography Inulin Enzyme assay chemistry.chemical_compound Enzyme Biochemistry Yield (chemistry) Materials Chemistry biology.protein Peptide sequence
Zdroj:	Carbohydrate Polymers. 66:75-80
ISSN:	0144-8617
DOI:	10.1016/j.carbpol.2006.02.027
Popis:	An inulin fructotransferase (DFA III-producing) [EC 2.4.1.93] from Leifsonia sp. T88-4 was purified and characterized, for the first time. The enzyme was purified from culture supernatant of the microorganism 32.4-fold with a yield of 11.3% using a DEAE–Toyopearl chromatography and two times of Super Q-Toyopearl chromatography. The enzyme showed maximum activity at pH 5.0 and 65 °C. This maximum temperature was highest in the inulin fructotransferase (DFA III-producing) reported to date. The enzyme activity was stable up to 60 °C after 30 min heat treatment. The molecular mass of the enzyme was estimated to be 44 kDa by SDS–PAGE and 74 kDa by gel filtration, and was considered to be a dimer. The N-terminal amino acid sequence (16 amino acid residues) was determined as METNVYDVTDVGVPGR. The Km value of the reaction at pH 5.0 and 60 °C was estimated to be 1.0 mM.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::784835b117fb19c3179f9bd9c7951599 https://doi.org/10.1016/j.carbpol.2006.02.027 Zobrazit plný text záznamu Full Text from ScienceDirect