Effects of Apigenin, Luteolin, and Quercetin on the Natural Killer (NK-92) Cells Proliferation: A Potential Role as Immunomodulator

Autor:	MYO OO@MOHD HASYM AUNG, NASIR MAT NOR, LIYANA HAZWANI MOHD ADNAN, NOR ZIDAH BINTI AHMAD, ABDI WIRA SEPTAMA, NIK NURUL NAJIHAH NIK NURUL NAJIHAH, MAR LWIN OHN, NORDIN SIMBAK
Rok vydání:	2021
Předmět:	chemistry.chemical_classification Multidisciplinary 05 social sciences Flavonoid Pharmacology chemistry.chemical_compound Tissue culture chemistry Cell culture 0502 economics and business Apigenin 050211 marketing Viability assay Quercetin Luteolin 050203 business & management Fetal bovine serum
Zdroj:	Sains Malaysiana. 50:821-828
ISSN:	2735-0118 0126-6039
DOI:	10.17576/jsm-2021-5003-22
Popis:	Cancer can be classified as a fourth leading cause of death in Malaysia. There is a continuous effort by scientists in finding alternative cure to cancer due to the known side effects of chemotherapy and radiation therapy as well as recurrences. One of the latest methods to kill cancerous cells is by using immune cells known as natural killer (NK) cells. Flavonoids such as flavone and flavonol are also known for their antioxidant, anti-inflammatory, immunomodulatory and anticancer properties. This study was carried out to determine the role of flavonoid compounds of apigenin, luteolin, and quercetin to facilitate the growth of NK-92 cells. NK-92 cell line was grown in tissue culture flasks containing α- Minimum Essential Medium (MEM) medium enriched with L-glutamine, 12.5% fetal bovine serum, 12.5% horse serum, 0.2 mM myo-inositol, 0.02 mM folic acid, and 100 - 200 U/mL recombinant interleukin 2 (IL-2). The cell viability was determined via trypan blue staining where the cells were manually counted by a haemocytometer. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cell viability of NK-92 cells after treatment with apigenin, luteolin, and quercetin. Results showed a dose-dependent proliferative effects of apigenin, luteolin, and quercetin on the proliferation of NK-92 cells showing the highest percentage of proliferation at 100 µg/mL for all compounds (*P < 0.05). However, exceeding the dose of 100 µg/mL had resulted in a decline of cell proliferations percentage. Based on these findings, flavonoid compounds comprising apigenin, luteolin and quercetin were able to induce proliferative effects on NK-92 cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::784318cfb8c291919261630ada59205e https://doi.org/10.17576/jsm-2021-5003-22 Zobrazit plný text záznamu