New optical methods for detecting monoamine neuromodulators
| Autor: | Jiesi Feng, Yulong Li, Jinxia Wan, Fangmiao Sun, Jianzhi Zeng |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Cell signaling Biomedical Engineering Medicine (miscellaneous) Bioengineering 02 engineering and technology Tyramine 021001 nanoscience & nanotechnology Biomaterials 03 medical and health sciences chemistry.chemical_compound Norepinephrine Monoamine neurotransmitter chemistry Dopamine medicine Octopamine (neurotransmitter) Serotonin 0210 nano-technology Neuroscience 030304 developmental biology medicine.drug G protein-coupled receptor |
| Zdroj: | Current Opinion in Biomedical Engineering. 12:68-74 |
| ISSN: | 2468-4511 |
| Popis: | Monoamine neuromodulators such as dopamine (DA), norepinephrine (NE), serotonin (5-HT), octopamine, and tyramine are signaling molecules in the nervous system, where they play critical roles in both health and disease. Given the complex spatio-temporal dynamics, similar structural features, and different receptors, studying their dynamics has been limited using conventional methods such as microdialysis and electrochemistry. However, recent advances in optics have facilitated the development of imaging-based detection methods. In this review, we summarize current detecting approaches for specific monoamines, emphasizing their design strategies, detection properties, applications, and limitations. We highlight the genetically encoded GPCR-based sensors for DA and NE, which have a high signal-to-noise ratio and selectivity, and can be used in vivo in different species. Finally, we discuss the potential for using this approach to generate new neuromodulator sensors with non-overlapping spectra, which will ultimately pave the way for studying the interplay between various neuromodulators and neurotransmitters. |
| Databáze: | OpenAIRE |
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