Ultra-Low-Volume, Real-Time Measurements of Lactate from the Single Heart Cell Using Microsystems Technology
| Autor: | Norbert Klauke, Godfrey L. Smith, Peter H. Cobbold, Xinxia Cai, Andrew Glidle, Jonathan M. Cooper |
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| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | Analytical Chemistry. 74:908-914 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac010941+ |
| Popis: | The fabrication of microelectrodes integrated within ultra-low-volume microtiter chambers for the amperometric determination of metabolites continues to be of interest in the subject of single-cell and high-throughput screening. The microsystem described in this paper consists of a two-microelectrode sensor with a microfluidic dispensation technology, which is able to deliver both very low titers (6.5 pL) and single heart cells into a low-volume microphotoelectrochemical cell. Devices were fabricated using photolithography and liftoff giving reproducible sensors integrated within high aspect ratio titer chambers (with a volume of 360 pL), made of the photoepoxy SU8. In this paper, the determination of lactate was optimized using an enzyme-linked assay based upon lactate oxidase, involving the amperometric determination of hydrogen peroxide at +640 mV versus an internal Ag|AgCl pseudoreference. The microsystem (including the microfluidic dispensers and structures as well as the microsensor) was subsequentl... |
| Databáze: | OpenAIRE |
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