| Autor: |
Christelle Grisez, Leslie Bottari, Françoise Prévot, Jean-Pierre Alzieu, Emmanuel Liénard, Fabien Corbière, Marie Rameil, Xavier Desclaux, Christophe Lacz, Christian Boulon, Julie Petermann, Jeanne Le Mével, Carine Vilardell, Philippe Jacquiet |
| Rok vydání: |
2020 |
| DOI: |
10.21203/rs.3.rs-37696/v1 |
| Popis: |
Background Bovine besnoitiosis is an emerging vector-borne disease in Europe caused by the Apicomplexa Besnoitia besnoiti. The mechanical transmission from infected to naïve hosts is permitted by horse flies and stable flies. Bovine besnoitiosis is difficult to control due to the complexity of its diagnosis in the acute stage of the disease, poor treatment success and chronically asymptomatic cattle acting as parasite reservoirs. When serological prevalence is low, detection and specific culling of seropositive cattle is feasible; however, economic considerations preclude this approach when serological prevalence is high. The aims of this study were to evaluate the accuracy of detection of super-spreaders in highly infected herds and to test their selective elimination as a new control strategy for bovine besnoitiosis. Methods Real-time PCR analyses performed on skin tissues from 160 asymptomatic animals sampled at slaughterhouses showed that the tail base was the best location to evaluate the dermal parasite DNA load. All seropositive animals (N = 518) from eight dairy or beef cattle farms facing a high serological prevalence of besnoitiosis were sampled and their skin analysed by real-time PCR. A recommendation of rapid and selective culling of super-spreaders was formulated and provided to the cattle breeders. Subsequent serological monitoring of naïve animals was used to evaluate the interest of this control strategy over time. Results Among the 518 seropositive animals, a low proportion of individuals (13%) showed Ct values below 36, 17% had doubtful results (36 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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