De novo transcriptome sequencing of the thecate colonial hydrozoan, Dynamena pumila
| Autor: | E.A. Konorov, Daria M. Kupaeva, S. V. Kremnyov |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences Contig cDNA library Morphogenesis Wnt signaling pathway Aquatic Science Biology 010603 evolutionary biology 01 natural sciences Transcriptome 03 medical and health sciences Sister group Evolutionary biology Genetics Gene Dynamena pumila 030304 developmental biology |
| Zdroj: | Marine Genomics. 51:100726 |
| ISSN: | 1874-7787 |
| DOI: | 10.1016/j.margen.2019.100726 |
| Popis: | As the sister group to bilaterian animals, cnidarians are very valuable models for comparative transcriptomics, shedding light on the evolution and genetic regulation of developmental trajectories, morphogenesis, body patterning and regeneration. To gain deeper insights into the evolution of developmental processes, wider sampling of biological diversity should be performed. In this study, we report the sequencing, assembly and analysis of the transcriptome for the first sertulariid hydrozoan, Dynamena pumila. We sequenced a mixed cDNA library from several developmental stages using Illumina HiSeq 4000. These sequences were assembled into 76,196 contigs with an N50 of 1890 bp. The pool of assembled sequences represents a wide variety of genes expressed during Dynamena development. Functional annotation with Gene Ontology and KEGG pathway analysis has been performed. With this, we identified Dynamena transcripts corresponding to the genes involved in major developmental signalling pathways, including Wnt and TGF-β. The data obtained contribute to the further development of evolutionary and functional transcriptomics and provide the reference transcriptome for future studies. |
| Databáze: | OpenAIRE |
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