Identification of Chelonid herpesvirus 5 (ChHV5) in endangered green turtles (Chelonia mydas) with fibropapillomatosis in Asia
| Autor: | Chao-Chin Chang, Thierry M. Work, Wei-Li Hsu, Yu-Ching Lan, Cheng-Tsung Tseng, Tsung-Hsien Li, George H. Balazs |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Fibropapillomatosis
Phylogenetic tree 040301 veterinary sciences Intranuclear Inclusions 0402 animal and dairy science Endangered species Zoology Molecular evidence 04 agricultural and veterinary sciences Aquatic Science Histopathological examination Biology Oceanography 040201 dairy & animal science 0403 veterinary science Panzootic |
| Zdroj: | Bulletin of Marine Science. 93:1011-1022 |
| ISSN: | 0007-4977 |
| DOI: | 10.5343/bms.2017.1018 |
| Popis: | Fibropapillomatosis (FP), a debilitating tumor disease of sea turtles, was first identified in green turtles [Chelonia mydas (Linnaeus, 1758)] in Florida in 1938. In recent decades, FP has been observed globally and is an emerging panzootic disease in sea turtles. However, few reports of FP in Asia exist. Here, we provide the first evidence of Chelonid herpesvirus 5 (ChHV5) DNA associated with FP in endangered green turtles from Taiwan, through molecular characterization, phylogenetic analysis, and histopathological examination. In our study, ChHV5 was successfully detected by PCR in the FP tumor lesions of green turtles. The sequences were found to be consistent with those of tumor-inducing viruses shown to affect sea turtles in the other parts of the world. ChHV5 RNA from the FP tissues was further detected by RT-PCR, indicating active replication of the viruses inside FP tumors. In addition to the molecular evidence of ChHV5 in FP, epidermal intranuclear inclusions were identified in tumor lesions upon histopathological examination. This further suggests that ChHV5 should be in a transcriptionally active (i.e., non-latent) state in FP tumors of affected green turtles. The phylogenetic tree revealed that ChHV5 from the green turtles in Taiwan were closest to the ChHV5 from Hawaii, Puerto Rico, and Sao Tome. For conservation of endangered sea turtles, ChHV5 should be considered an emerging virus, which threatens sea turtles in marine waters in Asia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|