Analysis of two sampling treatments of beef for microbiome studies based on metataxonomic

Autor: Otávio G Almeida, Caio F Freitas, Lara M V F Carvalho, Elaine C P De Martinis, Luis A Nero
Rok vydání: 2023
Předmět:
Zdroj: Letters in Applied Microbiology. 76
ISSN: 1472-765X
Popis: Microbial enumeration by serial dilution is one of the best resources to estimate cellular density for microbiological analysis. However, for metataxonomic analysis, it is not clear if serially diluted samples may accurately be used for metataxonomic analysis to represent species composition in beef samples. In this study, the effect of sampling preparation of beef samples on the bacterial composition was evaluated by the comparison of dilution and exudate. Based on the obtained results, data obtained from the exudate of the samples were more robust in terms of number of generated reads, but no significant differences in terms of biological diversity were observed (P
Databáze: OpenAIRE