| Popis: |
Plant tissue culture is one of the methods of plant propagation. Production costs in this method are relatively high. These costs include items such as the cost of culture vessels. This research aimed to investigate the replacement of new containers made of Cast Polypropylene (CPP) plastics instead of glass. For this study Petunia plant was selected as a model plant. In the first, all plants were disinfected. Then they were cultured in MS medium containing different amounts of 0, 0.5, 0.8, and 1 mg / l BAP which were made in different cultures of jam glasses, Test tubes, and CPP plastics containers. After establishment and proliferation stages, plants were transferred to a rooting medium supplemented by different concentrations of IBA and after 30 days, growth different factors were measured. The highest number and length of shoots and the highest number of leaves were obtained in 0.8 mg/l BAP culture medium in CPP plastic containers and the lowest amount was in a test tube culture media. The effect of regulator amount and container type in the rooting stage was not significant. |
