| Autor: |
Hai-tao Zhang, En-dong Bao, Xiao-dong Wu, Zhiliang Wang, Yutian Liu, Yongqiang Zhang |
| Rok vydání: |
2008 |
| Předmět: |
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| Zdroj: |
Agricultural Sciences in China. 7:1516-1523 |
| ISSN: |
1671-2927 |
| DOI: |
10.1016/s1671-2927(08)60411-3 |
| Popis: |
The method of immunohistochemistry assay for the detection of scrapie in Chinese Short-tailed Han sheep was established using monoclonal antibody. Genomic DNA was isolated from Chinese Short-tailed Han sheep blood. Using the polymerase chain reaction technique, PrP27-30 gene sequence was amplified from Chinese Short-tailed Han sheep genomic DNA. By recombinant DNA technology, the recombinant protein of Chinese Short-tailed Han sheep PrP27-30 was obtained. Then, using standard methodology of myeloma cell fusion, a panel of monoclonal antibodies was generated. With mAbs, scrapie in Chinese Short-tailed Han sheep was detected by immunohistochemistry assay. The recombinant protein of Chinese Short-tailed Han sheep PrP27-30 was obtained and a panel of six hybridoma cell lines secreting specific antibodies to Chinese Short-tailed Han sheep PrP27-30 related to scrapie was obtained with one fusion between myeloma Sp2/0 and spleen cells from mice immunized with the purified recombinant protein. Four hybridoma cell lines can be used in immunohistochemistry assay for the detection of scrapie in Chinese Short-tailed Han sheep. So that the special monoclonal antibody developed in author's institute can be used to detect PrPSc of scrapie in Chinese Short-tailed Han sheep by immunohistochemistry in China. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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