Differential Regulation of Stromelysin-1 (Matrix Metalloproteinase-3) and Matrilysin (Matrix Metalloproteinase-7) in Baboon Endometrium
| Autor: | A. T. Fazleabas, N. Kamiya, Kathryn E. Cox, K. M. Donnelly, M. Saraf, Kathy L. Sharpe-Timms |
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| Rok vydání: | 2000 |
| Předmět: |
0301 basic medicine
Matrix Metalloproteinase 3 medicine.medical_specialty Stromal cell 030102 biochemistry & molecular biology biology Obstetrics and Gynecology 030206 dentistry Matrix metalloproteinase Endometrium Stromelysin 1 03 medical and health sciences 0302 clinical medicine Endocrinology medicine.anatomical_structure biology.animal Internal medicine Gene expression medicine Matrilysin Baboon |
| Zdroj: | Journal of the Society for Gynecologic Investigation. 7:242-248 |
| ISSN: | 1071-5576 |
| DOI: | 10.1177/107155760000700409 |
| Popis: | To investigate in vivo expression of matrix metalloproteinase enzymes (MMP), MMP-3 and MMP-7, by the baboon endometrium in relation to the process of tissue remodeling that accompanies mensttruation in the primate. Endometrial tissues and uterine fluids obtained from reproductively intact cycling baboons, cycling baboons treated with an anti-progestin, and ovariectomized steroid-treated baboons were evaluated for MMP mRNA expression and protein production by using multiple analytic techniques. The pattern of MMP protein production matched the pattern of MMP mRNA expression. In intact cyclcing baboons, MMP mRNA expression and protein production were specific to cell type and stage of the menstrual cycle. Endometrial stroma expressed minimal amounts of MMP-3 during the proliferative and secretory stages, whereas endomterial epithelia expressed MMP-7 during the prolfierative stage. Expression of stromal MMP-3 and epithelial MMP-7 increased during early menses. Administration of anti-progestin beginning on the day of the LH surge increased MMP-7 but not MMP-3 expression at the mid secretory phase. Expression of MMP-3 and MMP-7 in the ovariectomized steroid-treated animals paralleled that of the intact cycling animals. These results highlight the similarity of human and baboon endometrial MMP-3 and MMP-7 production, providing further evidence for use of the baboon as a model for studying events associated with hormonally regulated chanes during the menstrual cycle. These results also demonstrate that ednometrial epithelial MMP-7 is suppressed by progresterone in the baboon endometrium, but endometrial stromal MMP-3 is regulated by a different mechanism. |
| Databáze: | OpenAIRE |
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