| Autor: |
J. Rule, Sandra Chuppa, Konstantin Konstantinov, S. Park, H. Qi, Rüdiger Heidemann, Chun Zhang, Jim Michaels, Carl Rozales, Manas K. Ray, David Naveh |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
Animal Cell Technology: Products from Cells, Cells as Products ISBN: 9780792360759 |
| Popis: |
This paper describes the test of several new vegetarian hydrolysates (peptones of soy, rice, wheat gluten etc.) as protein-free medium supplements for the production of a recombinant therapeutic protein. Multiple peptone-supplemented, continuous perfusion bioreactor experiments were conducted. Cell specific rates and product quality studies were obtained for the various peptones and compared with peptone-free medium. It was found that peptones confer a nutritional benefit, especially at low dilution rates, for a recombinant BHK cell line used in this investigation. The specific productivity increased 20–30% compared to the peptone-free controls. However, this benefit was also fully delivered by using fortified medium in place of the peptone-enriched media. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
