The chromosomal distribution of cloned highly repetitive sequences from hexaploid wheat

Autor:	D M Lonsdale, J Hutchinson
Rok vydání:	1982
Předmět:	Genetics Secale biology Heterochromatin food and beverages Chromosome Molecular cloning biology.organism_classification Genome Centromere Hordeum vulgare Repeated sequence Genetics (clinical)
Zdroj:	Heredity. 48:371-376
ISSN:	1365-2540 0018-067X
DOI:	10.1038/hdy.1982.49
Popis:	Two highly repetitive DNA sequences from Triticum aestivum were isolated and purified by molecular cloning. In situ hybridisation of these sequences to wheat chromosomes showed the sequence to be located predominantly at specific sites in the B genome and also chromosome 4A. Individual chromosomes could be identified by their patterns of labelling. Although one sequence was found to be mostly located close to centromeres and interstitially, and the other sequence mostly at telomeres, there was a good correlation between the distribution of these sequences and the distribution of heterochromatin as revealed by C-banding. In situ hybridisation of the two sequences to chromosomes of the related species, Secale cereale and Hordeum vulgare showed a similar correlation between the location of highly repetitive sequences and heterochromatin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::77d7ee812d13823035f94a9f9b6d937d https://doi.org/10.1038/hdy.1982.49 Zobrazit plný text záznamu