Curcumin prevents corticosterone-induced neurotoxicity and abnormalities of neuroplasticity via 5-HT receptor pathway

Autor: Jie Yu, Abhinav P. Acharya, Erin R. Boykin, Jianchun Pan, William O. Ogle, Ling Chen, Xiaoyu Pan, Ying Xu, Matthew M. Vernon, Subramaniam S. Govindarajan, Yuan Zhang, Shan Li, Philip A. Barish, James M. O'Donnell
Rok vydání: 2011
Předmět:
Zdroj: Journal of Neurochemistry. 118:784-795
ISSN: 0022-3042
DOI: 10.1111/j.1471-4159.2011.07356.x
Popis: J. Neurochem. (2011) 118, 784–795. Abstract Curcumin, a major active component of Curcuma longa, possesses antioxidant and neuroprotective activities. The present study explores the mechanisms underlying the neuroprotective effect of curcumin against corticosterone and its relation to 5-hydroxy tryptamine (5-HT) receptors. Exposure of cortical neurons to corticosterone results in decreased mRNA levels for three 5-HT receptor subtypes, 5-HT1A, 5-HT2A and 5-HT4, but 5-HT1B, 5-HT2B, 5-HT2C, 5-HT6 and 5-HT7 receptors remain unchanged. Pre-treatment with curcumin reversed this effect on mRNA for the 5-HT1A and 5-HT4 receptors, but not for the 5-HT2A receptor. Moreover, curcumin exerted a neuroprotective effect against corticosterone-induced neuronal death. This observed effect of curcumin was partially blocked by either 5-HT1A receptor antagonist p-MPPI or 5-HT4 receptor antagonist RS 39604 alone; whereas, the simultaneous application of both antagonists completely reversed the effect. Curcumin was also found to regulate corticosterone-induced morphological changes such as increases in soma size, dendritic branching and dendritic spine density, as well as elevate synaptophysin expression in cortical neurons. p-MPPI and RS 39604 reversed the effect of curcumin-induced change in neuronal morphology and synaptophysin expression of corticosterone-treated neurons. In addition, an increase in cyclic adenosine monophosphate (cAMP) level was observed after curcumin treatment, which was further prevented by RS 39604, but not by p-MPPI. However, curcumin-induced elevation in protein kinase A activity and phosphorylation of cAMP response element-binding protein levels were inhibited by both p-MPPI and RS 39604. These findings suggest that the neuroprotection and modulation of neuroplasticity exhibited by curcumin might be mediated, at least in part, via the 5-HT receptor-cAMP-PKA-CREB signal pathway.
Databáze: OpenAIRE
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