Abstract 4740: Evaluation of highly-multiplexed custom panels for targeted massively parallel semiconductor sequencing on paraffin DNA
| Autor: | Elpida Charalambous, Aggeliki Lyberopoulou, Eleftheria Tsolaki, George Fountzilas, Kyriaki Papadopoulou, Vassiliki Kotoula, Zoi Alexopoulou, Konstantinos Lilakos, Sotiris Lakis |
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| Rok vydání: | 2015 |
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| Zdroj: | Cancer Research. 75:4740-4740 |
| ISSN: | 1538-7445 0008-5472 |
| DOI: | 10.1158/1538-7445.am2015-4740 |
| Popis: | Background: Targeted massively parallel sequencing (MPS) is increasingly applied for tumor genotyping on routinely processed paraffin tissues, mostly with commercially available, predesigned multigene panels. In comparison, custom panels remain poorly addressed, despite that this approach would increase genotyping flexibility, especially in translational research in Oncology. Methods: Two custom multigene panels (B, 373 amplicons; T, 286 amplicons) were designed and evaluated with semiconductor sequencing on paired DNA sample series from 158 paraffin breast tumors and 133 matched peripheral blood samples (total n samples = 316, total n libraries = 332). The two panels shared 26.6% targeted regions (common or shifted amplicons). Amplicon performance and variant (coding and non-coding) concordance were evaluated in paired groups of samples and datasets. Results: Amplicon read ratios strongly correlated in blood and paraffin samples with Spearman's rho = 0.6055 for the B- and 0.8212 for the T-panel (both p's 75 and Citation Format: Vassiliki Kotoula, Aggeliki Lyberopoulou, Kyriaki Papadopoulou, Elpida Charalambous, Sotiris Lakis, Zoi Alexopoulou, Eleftheria Tsolaki, Konstantinos Lilakos, George Fountzilas. Evaluation of highly-multiplexed custom panels for targeted massively parallel semiconductor sequencing on paraffin DNA. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 4740. doi:10.1158/1538-7445.AM2015-4740 |
| Databáze: | OpenAIRE |
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