Characterization of Natural Product Biosynthetic Pathways by In Vitro Reconstitution

Autor:	Chaitan Khosla, Jake Hsu
Rok vydání:	2020
Předmět:	chemistry.chemical_classification chemistry.chemical_compound Natural product Enzyme chemistry Biochemistry Biosynthesis In vivo Context (language use) Biological activity Heterologous expression In vitro
DOI:	10.1016/b978-0-12-409547-2.14726-2
Popis:	Natural product pathways are remarkable for their ability to produce medicines and other compounds with useful biological activity. Although methods such as native host cultivation and in vivo heterologous expression are powerful techniques in understanding biosynthesis, in vitro reconstitution of the pathway offers distinct advantages in elucidating precise details such as enzyme substrate specificity and intermediate steps of product formation. Here, we provide an overview of how in vitro reconstitution has been utilized to elucidate mechanisms of natural product biosynthesis and discuss its future outlook in the context of biosynthetic pathway characterization.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::77c45de353972644c5af206c29c978cb https://doi.org/10.1016/b978-0-12-409547-2.14726-2 Zobrazit plný text záznamu