An overlooked hybrid between the two diploid Chenopodium species in Central Europe determined by microsatellite and morphological analysis
Autor: | Eva Hodková, Bohumil Mandák |
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Rok vydání: | 2017 |
Předmět: |
0106 biological sciences
biology Chenopodium Plant Science biology.organism_classification 010603 evolutionary biology 01 natural sciences Taxon Evolutionary biology Genetic marker Morphological analysis Backcrossing Microsatellite Ploidy Ecology Evolution Behavior and Systematics 010606 plant biology & botany Hybrid |
Zdroj: | Plant Systematics and Evolution. 304:295-312 |
ISSN: | 2199-6881 0378-2697 |
Popis: | The presence and extent of hybridization within the Chenopodium album aggregate (Amaranthaceae) is still unclear. Although many hybrid combinations have been described, their existence in the field has never been systematically studied and verified. The main aim of this study was to ascertain the extent of interspecific hybridization between the diploid species C. ficifolium and C. suecicum using highly variable nuclear microsatellite markers. Due to the absence of such kind of molecular markers for the whole C. album group, we divided the analysis into two steps: (1) Eleven microsatellite loci designed for the closely related species C. quinoa were cross-amplified in five Eurasian species of the C. album diploid–polyploid complex, i.e. C. album s.s. (6x), C. striatiforme (4x), C. strictum (4x), C. ficifolium (2x) and C. suecicum (2x); (2) For the detection of interspecific hybridization between C. ficifolium and C. suecicum, we sampled 480 individuals from five localities in Central Europe. We also investigated morphological differences between the parental taxa and their hybrid and devised a key for their determination. Analysis of variation in microsatellite loci using Bayesian methods, PCoA and Neighbour-joining tree identified 32 F1 hybrids. These F1 hybrids, described here as C. paradoxum Mandak, formed a cluster between well-differentiated parental species, combining the morphological characters of both their parents. Moreover, genetic analyses also recognized several F2 or backcross hybrids, whose delimitation, mainly from C. suecicum and F1 hybrids, based on morphological characters, is problematic. |
Databáze: | OpenAIRE |
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