Microchip-based immunoassay system with branching multichannels for simultaneous determination of interferon-γ

Autor:	Manabu Tokeshi, Hiroko Takahashi, Takehiko Kitamori, Hiroko M. Kimura, Kiichi Sato, Maho Yamanaka
Rok vydání:	2002
Předmět:	Chromatography Microscope medicine.diagnostic_test Chemistry Clinical Biochemistry Microfluidics Detector Analytical chemistry Branching (polymer chemistry) Chip Biochemistry Signal Analytical Chemistry law.invention Interferon γ law Immunoassay medicine
Zdroj:	ELECTROPHORESIS. 23:734-739
ISSN:	1522-2683 0173-0835
DOI:	10.1002/1522-2683(200203)23:5<734::aid-elps734>3.0.co;2-w
Popis:	A bead-bed immunoassay system suitable for simultaneous assay of multiple samples was constructed on a microchip. The chip had branching multichannels and four reaction and detection regions; the constructed system could process four samples at a time with only one pump unit. Interferon γ was assayed by a 3-step sandwich immunoassay with the system coupled to a thermal lens microscope as a detector. The biases of the signal intensities obtained from each channel were within 10%, and coefficients of variation were almost the same level as the single straight channel assay. The assay time for four samples was 50 min instead of 35 min for one sample in the single-channel assay; hence higher throughput was realized with the branching structure chip.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::77b721e0edd94e991cbcc97338551bba https://doi.org/10.1002/1522-2683(200203)23:5<734::aid-elps734>3.0.co Zobrazit plný text záznamu Plný text