Immobilization of uricase on ZnO nanorods for a reagentless uric acid biosensor

Autor:	Yuezhong Xian, Xiao-Li Wang, Zi-Qiang Zhu, Katsunobu Yamamoto, Qiao Wan, Fen-Fen Zhang, Litong Jin, Zhengdong Sun, Shiyun Ai
Rok vydání:	2004
Předmět:	Detection limit Inorganic chemistry Urate oxidase Electrocatalyst Biochemistry Analytical Chemistry chemistry.chemical_compound Electron transfer chemistry Environmental Chemistry Uric acid Thermal stability Nanorod Biosensor Spectroscopy
Zdroj:	Analytica Chimica Acta. 519:155-160
ISSN:	0003-2670
DOI:	10.1016/j.aca.2004.05.070
Popis:	A reagentless uric acid (UA) biosensor based on uricase immobilized on ZnO nanorods was developed. Direct electrochemistry and thermal stability of immobilized uricase were studied. The ZnO nanorods derived electrode retained the enzyme bioactivity and could enhance the electron transfer between the enzyme and the electrode. This sensor showed a high thermal stability up to 85 °C and an electrocatalytic activity to the oxidation of uric acid without the presence of an electron mediator. The electrocatalytic response showed a linear dependence on the uric acid concentration ranging from 5.0 × 10−6 to 1.0 × 10−3 mol L−1 with a detection limit of 2.0 × 10−6 mol L−1 at 3σ. The apparent KMapp value for the uric acid sensor was estimated to be 0.238 mM, showing a high affinity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::77985289f8024583c1cf4a59a05225cd https://doi.org/10.1016/j.aca.2004.05.070 Zobrazit plný text záznamu Full Text from ScienceDirect