Enhancement of L-Threonine Production by Controlling Sequential Carbon-Nitrogen Ratios during Fermentation
| Autor: | Hee Suk Lee, Jin Gyeom Lee, Eun Gyo Lee, Hyeok Won Lee, Won-Kyo Kim, Chun Suk Kim, Hongweon Lee |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Industrial fermentation General Medicine Applied Microbiology and Biotechnology Water consumption 03 medical and health sciences Ammonia chemistry.chemical_compound 030104 developmental biology chemistry Carbon nitrogen SCALE-UP Production (economics) Fermentation Food science Biotechnology L-threonine |
| Zdroj: | Journal of Microbiology and Biotechnology. 28:293-297 |
| ISSN: | 1738-8872 1017-7825 |
| DOI: | 10.4014/jmb.1708.08072 |
| Popis: | Controlling the residual glucose concentration is important for improving productivity in L-threonine fermentation. In this study, we developed a procedure to automatically control the feeding quantity of glucose solution as a function of ammonia-water consumption rate. The feeding ratio (RC/N) of glucose and ammonia water was predetermined via a stoichiometric approach, on the basis of glucose-ammonia water consumption rates. In a 5-L fermenter, 102 g/l L-threonine was obtained using our glucose-ammonia water combined feeding strategy, which was then successfully applied in a 500-L fermenter (89 g/l). Therefore, we conclude that an automatic combination feeding strategy is suitable for improving L-threonine production. |
| Databáze: | OpenAIRE |
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