Effects of Hemoglobin C and S Traits on Eight Glycohemoglobin Methods
| Autor: | Randie R. Little, Ryan B. Lundell, Curt Rohlfing, Barun K. De, C. Michael Hanbury, Thomas L. Lambert, William L. Roberts, Diane Brown, James D. Hoyer, W. Garry John |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
business.industry Biochemistry (medical) Clinical Biochemistry Adult population medicine.disease Laboratory results Diabetes Control and Complications Trial Surgery Hemoglobin C Endocrinology Hemoglobinopathy Internal medicine Diabetes mellitus medicine Hemoglobin business Glycemic |
| Zdroj: | Clinical Chemistry. 48:383-385 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/48.2.383 |
| Popis: | Glycohemoglobin (gHb) is a marker of long-term glycemic control that has been shown to correlate with complications of diabetes mellitus (1). The National Glycohemoglobin Standardization Program (NGSP) was established to standardize gHb results so that clinical laboratory results are comparable to those reported by the Diabetes Control and Complications Trial (2)(3). Previous studies have shown that some gHb methods yield inaccurate results with samples heterozygous for hemoglobin (Hb) C or Hb S (4)(5)(6). At least 10% of black Americans have either Hb C or S trait, and there were 19 million black Americans over age 19 in 1990 (7)(8)(9)(10). The prevalence of diabetes is estimated to be 5.1% of the adult population, with the rate for non-Hispanic blacks being 1.6-fold higher than that of non-Hispanic whites (11). It is therefore probable that at least 150 000 Americans with diabetes have Hb C or S trait. Here, we investigate the measurement of gHb in specimens containing Hb C or S trait using eight gHb methods currently in clinical use. Whole blood samples from individuals homozygous for Hb A (n = 43) and heterozygous for Hb C or S (n = 43 and 61, respectively) were collected in EDTA tubes. Hb variants were identified by their characteristic Variant A1c HPLC chromatograms (Bio-Rad Laboratories). Aliquots of these samples containing between 4% and 14% Hb A1c (NGSP Hb A1c evaluation range) were stored refrigerated (2–8 °C) and analyzed within 10 days of collection. This study was approved by the Institutional Review Board of the University of Utah. The CLC 330 gHb analyzer (Primus Corporation) was operated at the University of Missouri. This method was chosen as the comparison method for this study because results from boronate affinity chromatography should not … |
| Databáze: | OpenAIRE |
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