Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment
| Autor: | Congqiang Luo, Chunlong Yi, Longgen Guo, Leyi Ni |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Chlorophyll a Biomass (ecology) Hypophthalmichthys 010504 meteorology & atmospheric sciences biology Ecology 010604 marine biology & hydrobiology fungi Zoology Bacterioplankton Oceanography biology.organism_classification 01 natural sciences Zooplankton Mesocosm chemistry.chemical_compound Cladocera chemistry Microbial loop 0105 earth and related environmental sciences Water Science and Technology |
| Zdroj: | Journal of Oceanology and Limnology. 36:341-350 |
| ISSN: | 2523-3521 2096-5508 |
| Popis: | We characterized variations in bacterioplankton community composition (BCC) in mesocosms subject to three different treatments. Two groups contained fish (group one: Cyprinus carpio; group two: Hypophthalmichthys molitrix); and group three, the untreated mesocosm, was the control. Samples were taken seven times over a 49-d period, and BCC was analyzed by PCR-denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR (qPCR). Results revealed that introduction of C. carpio and H. molitrix had a remarkable impact on the composition of bacterioplankton communities, and the BCC was significantly different between each treatment. Sequencing of DGGE bands revealed that the bacterioplankton community in the different treatment groups was consistent at a taxonomic level, but differed in its abundance. H. molitrix promoted the richness of Alphaproteobacteria and Actinobacteria, while more bands affiliated to Cyanobacteria were detected in C. carpio mesocosms. The redundancy analysis (RDA) result demonstrated that the BCC was closely related to the bottom-up (total phosphorus, chlorophyll a, phytoplankton biomass) and top-down forces (biomass of copepods and cladocera) in C. carpio and control mesocosms, respectively. We found no evidence for top-down regulation of BCC by zooplankton in H. molitrix mesocosms, while grazing by protozoa (heterotrophic nanoflagellates, ciliates) became the major way to regulate BCC. Total bacterioplankton abundances were significantly higher in C. carpio mesocosms because of high nutrient concentration and suspended solids. Our study provided insights into the relationship between fish and bacterioplankton at species level, leading to a deep understanding of the function of the microbial loop and the aquatic ecosystem. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|