Osteochondral Allografts With an Intramedullary Muscle Flap in Rabbits
Autor: | Roger K. Khouri, Michael W. Vannier, David M. Brown, Sampath Tk, Lantieri La, N. M. Kania, Chung Sh, Hodge Jc |
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Rok vydání: | 1997 |
Předmět: |
Pathology
medicine.medical_specialty education.field_of_study Medullary cavity Callus formation business.industry Population chemical and pharmacologic phenomena General Medicine Anatomy law.invention Intramedullary rod surgical procedures operative medicine.anatomical_structure Vascularity law medicine Orthopedics and Sports Medicine Surgery Shoulder joint Humerus medicine.symptom Surgical Flaps education business |
Zdroj: | Clinical Orthopaedics and Related Research. 334:282 |
ISSN: | 0009-921X |
Popis: | A model for hemijoint reconstruction using partially demineralized and lyophilized osteochondral allografts combined with an intramedullary muscle flap is described. The proximal 2/3 of the humerus was resected in 10 rabbits. The remaining defect was reconstructed with either a control lyophilized osteochondral allograft or a lyophilized allograft with a muscle flap filling the marrow cavity. Graft healing was followed by serial radiographs and magnetic resonance imaging. The grafts were harvested at 5 weeks for histologic analysis. By 5 weeks, 4 of 5 control allografts had fractured. In contrast, only 1 allograft with an intramedullary muscle flap showed evidence of a cortical break. Magnetic resonance imaging of control allografts showed a persistent large dead space within the marrow cavity and callus formation only at the outer cortical surface. Magnetic resonance imaging of allografts with an intramedullary muscle flap showed muscle obliterating the marrow cavity and areas of callus formation at both the outer and inner cortical surfaces. Histologically, graft incorporation was occurring at the outer cortical surface of the control allografts. In contrast, graft incorporation was occurring at both the outer and inner cortical surfaces of the allografts with an intramedullary muscle flap. The articular surface of the control allografts was severely degenerated. In allografts with an intramedullary muscle flap, the articular surface was smoother. Joints reconstructed with allografts with an intramedullary muscle flap had a significantly better range of motion at 5 weeks compared with control allografts. These results suggest that an intramedullary muscle flap can improve the functional results of joints reconstructed with partially demineralized and lyophilized osteochondral allografts by providing both vascularity and an increased population of mesenchymal stem cells capable of responding to bone morphogenetic proteins that reside in the partially demineralized allograft. |
Databáze: | OpenAIRE |
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