Analysis of Urine to Monitor Exposures to Benzidine, o-Dianisidine, o-Tolidine, and 4, 4′-Methylenedianiline

Autor:	Charles E. Neumeister
Rok vydání:	1991
Předmět:	Hydrolysis chemistry.chemical_compound Sorbent Chromatography 4 4'-Methylenedianiline Chemistry Elution Public Health Environmental and Occupational Health Urine Acetonitrile Carcinogen Benzidine
Zdroj:	Applied Occupational and Environmental Hygiene. 6:953-958
ISSN:	1521-0898 1047-322X
Popis:	Aromatic diamines have great potential for dermal absorption as an exposure route. Also, the body exhibits the ability to reductively cleave an azo dye to its corresponding aromatic diamine. These factors have demonstrated the need for biological monitoring to complement environmental monitoring when assessing occupational exposures to these compounds. This is especially important since certain aromatic diamines are known or are potential human carcinogens. Therefore, a sampling and analytical procedure for the measurement of benzidine, o-dianisidine, o-tolidine, and 4, 4′-methylenedianiline in urine was developed for use in industrial hygiene investigations. After hydrolysis of urine metabolites, the free diamines are isolated using a C18 solid sorbent. The free diamines are eluted, concentrated, and injected into a high-performance liquid chromatography system. Separation is achieved using a C18 column with a mobile phase of acetonitrile and water. Identification and quantitation is achieved by...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::777e2560e5fe4867da2bbc8ac193ca16 https://doi.org/10.1080/1047322x.1991.10387997 Zobrazit plný text záznamu