Continuous monitoring of the activation and activity of 6NiFe9-hydrogenases by membrane-inlet mass spectrometry
| Autor: | E. Claude Hatchikian, Sylvie Elsen, Larissa T. Serebryakova, Laurent Cournac, Paulette M. Vignais, Bernard Dimon, Nikolay A. Zorin |
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| Rok vydání: | 2002 |
| Předmět: |
Rhodobacter
Hydrogenase biology Renewable Energy Sustainability and the Environment Chemistry Stereochemistry Synechocystis Energy Engineering and Power Technology Condensed Matter Physics biology.organism_classification Desulfovibrio Catalysis Fuel Technology Biochemistry Rhodospirillales Rhodospirillaceae Anabaena variabilis |
| Zdroj: | International Journal of Hydrogen Energy. 27:1441-1448 |
| ISSN: | 0360-3199 |
| Popis: | The hydrogen-deuterium (H + /D 2 ) exchange reaction catalyzed by [NiFe]-hydrogenases in the D 2 /H 2 O system has been used to study enzyme activation and activity by membrane-inlet mass spectrometry. The activation of the [NiFe]-hydrogenases from Thiocapsa roseopersicina (HynSL), Desulfovibrio fructosovorans (HynSL), Desulfomicrobium baculatum (HysSL), Rhodobacter capsulatus (HupUV), and of the bidirectional tetrameric HoxFUYH enzymes from Synechocystis PCC 6308 (Gloeocapsa alpicola) and Anabaena variabilis ATCC 29413 was determined in response to oxygen depletion and to reductant addition (molecular hydrogen, reduced methyl viologen). Natural physiological activators (NADH, NADPH) of the bidirectional [NiFe] hydrogenases could also be identified by the H + /D 2 exchange reaction. The data are discussed in the light of current models of hydrogenase catalytic mechanism. |
| Databáze: | OpenAIRE |
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