Encapsulation of Date Palm Somatic Embryos: Synthetic Seeds
| Autor: | Shawky A. Bekheet |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Germplasm Sucrose Somatic embryogenesis Gel matrix food and beverages Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Horticulture 030104 developmental biology chemistry Germination Offshoot Palm 010606 plant biology & botany Sodium alginate |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493971589 |
| DOI: | 10.1007/978-1-4939-7159-6_7 |
| Popis: | Synthetic seed or encapsulated somatic embryos may be used for propagation, storage, and exchange of plant germplasm and have many diverse applications in date palm cultivation. They have advantages over conventional use of offshoot material for germplasm propagation, maintenance, exchange, and transportation. This chapter describes a protocol for date palm synthetic seed production by encapsulation of somatic embryos with sodium alginate. Among three concentrations used, 3% sodium alginate followed by dropping into 2.5% calcium chloride (CaCl2) solution shows the best concentration of gel matrix for both maintenance and recovery. In addition, storage of the encapsulated date palm somatic embryos at 5 °C improves the survival and conversion into plantlets; otherwise, 20 g/L sucrose in the culture medium enhances conversion of the recovered somatic embryos to plantlets. This protocol is promising for in vitro conservation and international exchange of date palm germplasm. |
| Databáze: | OpenAIRE |
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