Effect of Extracellular Ca++ & Na+ on Intracellular pH in Human
| Autor: | Keizo Yamada, Hajime Tsuji, Toshiyuki Tamagaki, Yuka Miyashita, Osamu Takada, Takeo Toyoda, Kazuharu Kato, Mitsuru Yoneda, Shohei Sawada, Kaoru Shirai, Katsumi Nakagawa, Masao Nakagawa, Katsumi Yamamoto, Haruchika Masuda, Kyoichiro Kobayashi, Masahito Yamagami |
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| Rok vydání: | 1992 |
| Předmět: | |
| Zdroj: | Japanese Journal of Thrombosis and Hemostasis. 3:22-28 |
| ISSN: | 1880-8808 0915-7441 |
| DOI: | 10.2491/jjsth.3.22 |
| Popis: | The effect of extracellular Ca++ and Na+ on the intracellular pH ([pH]i) of human umbilical vein endothelial cells (HUVEC) was investigated using a fluorescent pH indicator, 2′, 7′-bis (carboxyethyl) carboxyfluorescein (BCECF) with reference to C-kinase and PGI2 generation. Thrombin increased PGI2 generation or intracellular Ca++ concentration ([Ca++]i). Pretreatment with EGTA or H-7 decreased thrombin-induced PGI2 generation or [Ca++]i, but did not affect on the baseline PGI2 generation and [Ca++]i. Na+-free buffer did not affect on the enhancement effect. Addition of Ca++ or thrombin alkalified [pH]i, and pretreatment with EGTA decreased basal or thrombin-induced alkalinization. Pretreatment with H-7 did not affect on the baseline [pH]i, but decreased thrombininduced alkalinization. Na+/H+ antiporter, amiloride remarkably decreased basal or thrombin-induced alkalinization. Nat-free buffer decreased both alkalinization. These results suggested that thrombin activated the Na+/H+ exchange system, and that this activation was mediated by an increase in [Ca++]i or by C-kinase. |
| Databáze: | OpenAIRE |
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