Cytotoxic effects of novel solvothermal synthesised Ag-doped PEGylated WO3 sheet-like nanocomposites on MCF-7 human breast cancer cells
| Autor: | Blassan P. George, Eric M. Ngigi, Heidi Abrahamse, Philiswa N. Nomngongo, Jane Catherine Ngila |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Solvothermal synthesis technology industry and agriculture Bioengineering 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Tungsten trioxide Atomic and Molecular Physics and Optics 0104 chemical sciences Nanomaterials chemistry.chemical_compound Hoechst stain chemistry MCF-7 Modeling and Simulation Cancer cell PEGylation General Materials Science 0210 nano-technology Cytotoxicity Nuclear chemistry |
| Zdroj: | Journal of Nanoparticle Research. 22 |
| ISSN: | 1572-896X 1388-0764 |
| DOI: | 10.1007/s11051-020-04929-0 |
| Popis: | Nanotechnology has provided an unprecedented opportunity in addressing challenges faced by cancer treatment. Nanomaterials represent a promising and versatile method in biomedical applications. PEGylation of nanomaterials is considered as the most effective strategy for commercial relevance. The purpose of this study was to develop a novel solvothermal synthesis of PEGylated tungsten trioxide (PEG-WO3) and Ag-doped PEGylated tungsten trioxide (Ag/PEG-WO3) (1:2 ratio) sheet-like nanocomposites and to investigate the in vitro anticancer activities of PEGylated WO3 and Ag-doped PEGylated tungsten trioxide (Ag/PEG-WO3) (1:2 ratio) nanoparticles on MCF-7 human breast cancer cells. The structural, morphological and textural properties were characterized by powder X-ray diffractometer, transmission electron microscope, UV-vis diffuse reflectance spectroscopy and FTIR. The XRD analysis proved that doping of Ag NPs into the lattice of PEGylated WO3 and TEM image indicated the successful anchoring of spherical-like shape of Ag NPs approximately 6–8 nm in size onto the sheet-like PEGylated WO3. Morphological changes, ATP proliferation, cytotoxicity, caspase activity and nuclear damage of NPs treated an MCF-7 cell line were assessed. The Ag-doped PEGylated WO3 nanoparticles (10 μg/mL) showed more toxicity towards MCF-7 cells in LDH assay (p |
| Databáze: | OpenAIRE |
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