| Autor: |
B.M. Siegel, William T. Freeman, Michael Isaacson, Earl J. Kirkland, M. Ohtsuki |
| Rok vydání: |
1981 |
| Předmět: |
|
| Zdroj: |
Ultramicroscopy. 6:367-376 |
| ISSN: |
0304-3991 |
| DOI: |
10.1016/s0304-3991(81)80223-9 |
| Popis: |
Computer image processing is applied to moderately low dose (11 e/A 2 ) STEM images of stained tobacco mosaic virus (TMV, intact helix). Periodic signal averaging and several simple methods of image enhancement are used. A method of separation of the three-dimensional structure of the TMV helix (top/bottom separation) is also presented and applied to the signal average image. TMV has previously been found (by X-ray diffraction) to be a single strand RNA helix with large protein subunits periodically attached. In the processed images the protein subunits are clearly visible in positions consistent with a helical structure of 49 subunits per three turns. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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