Preliminary TLC analysis of fructooligosaccharides as feed additives

Autor:	Katarína Reiffová, Andrej Oriňák, Tat’ána Gondová, Karol Flórián, Jana Podolonovičová
Rok vydání:	2003
Předmět:	Chloroform Chromatography Silica gel Clinical Biochemistry Maltodextrin Biochemistry Thin-layer chromatography Analytical Chemistry chemistry.chemical_compound chemistry Reagent Derivatization Phosphoric acid Sodium acetate
Zdroj:	Journal of Planar Chromatography – Modern TLC. 16:52-57
ISSN:	1789-0993 0933-4173
DOI:	10.1556/jpc.16.2003.1.11
Popis:	A rapid, simple qualitative thin-layer chromatographic method has been developed for separation of mono- and fructooligosaccharides from feed additives with the aim of analyzing them in biological materials. The fructooligosaccharides were analyzed in the commercially available dietetic feeding products Raftifeed and Raftilose (Orafti, Tienen, Belgium) and in the polysaccharide maltodextrin. Silica gel layers were pretreated either with sodium acetate or with a mixture of chloroform and methanol, and three different mobile phases were used. High-performance amino-bonded layers were developed with acetonitrile-water-phosphate buffer without previous pretreatment of the layers. Post-chromatographic derivatization of chromatograms was accomplished with dipheny-lamine-aniline-phosphoric acid reagent. Densitometric evaluation of chromatograms was performed at λ = 370 nm.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::7720c86b26e85b840687d5000e22e08a https://doi.org/10.1556/jpc.16.2003.1.11 Zobrazit plný text záznamu Full text from SpringerLink