Interactive effects of zinc and nickel on the glutathione system state in Mimulus guttatus plants
| Autor: | Vl. V. Kuznetsov, E. B. Bashmakova, N. L. Radyukina, Pavel P. Pashkovskiy |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
biology Glutathione reductase Plant physiology Plant Science Glutathione 010501 environmental sciences medicine.disease_cause 01 natural sciences Enzyme assay Lipid peroxidation chemistry.chemical_compound Glutathione S-transferase chemistry Biochemistry Shoot biology.protein medicine Food science Oxidative stress 010606 plant biology & botany 0105 earth and related environmental sciences |
| Zdroj: | Russian Journal of Plant Physiology. 63:626-635 |
| ISSN: | 1608-3407 1021-4437 |
| Popis: | To determine whether the enhanced stress tolerance of ZnSO4 with NiSO4-treated Mimulus guttatus Fischer ex DC. plants was associated with the glutathione (GR-GSH) system, we investigated the changes in glutathione redox state (reduced (GSH), oxidized (GSSG) forms, total reduced (GSHt) glutathione, and GSH/GSSG ratio) and in the enzymatic activities of glutathione reductase (GR) and peroxidatic glutathione S-transferases (GST). The 6-week-old plants were grown in water culture during 4 weeks on a modified Rorison’s medium with ZnSO4 (50, 100, and 200 μM) and NiSO4 (20 and 80 μM) in a condition of separate or simultaneous supply of the components. Dry biomass accumulations of roots and shoots were not influenced by the examined treatments. The positive correlations between the total external concentrations of ZnSO4 and NiSO4 and the total Zn and Ni contents in roots and leaves were found. It was determined that the MDA content was higher in the ZnSO4-treated plants than in the NiSO4-treated ones. The supplementation of the ZnSO4-treated plants with varied concentrations of NiSO4 decreased the Zn-induced increase in the MDA levels. The inverse proportionality between the MDA and pigment levels in leaves was found. The Zn-Ni interactions were shown to induce the decreases in the GR activity, the total peroxidatic GST activity, and the GSH/GSSG ratio in roots. However, in leaves, the GR activity and the GSH/GSSG ratio were significantly increased and the total peroxidatic GST activity was decreased. The supplementation of the ZnSO4-treated plants with varied concentrations of NiSO4 restored the Zn-induced reduction in the GSHt levels in roots and decreased the Zn-induced increase in the GSSG levels in leaves, which resulted in more reduced state of the intracellular environment. It was likely to cause a decrease of the MDA level. Thus, our studies on the Zn−Ni interactions identified the antagonizing role of Ni in Zn toxicity by the GR-GSH system. |
| Databáze: | OpenAIRE |
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