Identification of Campylobacter jejuni , C. coli , C. lari , C. upsaliensis , Arcobacter butzleri , and A. butzleri -Like Species Based on the glyA Gene

Autor:	Shahnaz T. Al Rashid, Voon Loong Chan, Wendy M. Johnson, David Ng, Irene Dakuna, Helena Louie, Peter Vandamme
Rok vydání:	2000
Předmět:	Microbiology (medical) Genetics biology Campylobacter Campylobacteraceae Lari biology.organism_classification medicine.disease_cause Campylobacter jejuni Microbiology Arcobacter butzleri law.invention genomic DNA law Arcobacter medicine Polymerase chain reaction
Zdroj:	Journal of Clinical Microbiology. 38:1488-1494
ISSN:	1098-660X 0095-1137
DOI:	10.1128/jcm.38.4.1488-1494.2000
Popis:	Currently, the detection and identification of Campylobacter and Arcobacter species remains arduous, largely due to cross-species phenotypic similarities and a relatively narrow spectrum of biochemical reactivity. We have developed a PCR-hybridization strategy, wherein degenerate primers are used to amplify glyA fragments from samples, which are then subjected to species-specific oligodeoxyribonucleotide probe hybridizations, to identify and distinguish between Campylobacter jejuni , C. coli , C. lari , C. upsaliensis , Arcobacter butzleri , and an A. butzleri -like species. Evaluation of this strategy with genomic DNA from different type strains suggests that this approach is both specific and sensitive and thus may be applicable in a diagnostic assay to identify and differentiate these highly related species.
Databáze:	OpenAIRE
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