Wiring and Molecular Features of Prefrontal Ensembles Representing Distinct Experiences
| Autor: | Ilana B. Witten, Joshua H. Jennings, Kimberly R. Thompson, William E. Allen, Alison L. Barth, Brian Hsueh, Karl Deisseroth, Liqun Luo, Casey H. Halpern, Qiyuan Tian, Ai-Chi Wang, Jennifer A. McNab, Li Ye, Avishek Adhikari, Charu Ramakrishnan |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adaptive behavior Cell type Molecular phenotype Biology Brain mapping General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Expression (architecture) Cellular logic Prefrontal cortex Neuroscience Basic Helix-Loop-Helix Transcription Factors 030217 neurology & neurosurgery |
| Zdroj: | Cell. 165:1776-1788 |
| ISSN: | 0092-8674 |
| Popis: | A major challenge in understanding the cellular diversity of the brain has been linking activity during behavior with standard cellular typology. For example, it has not been possible to determine whether principal neurons in prefrontal cortex active during distinct experiences represent separable cell types, and it is not known whether these differentially active cells exert distinct causal influences on behavior. Here, we develop quantitative hydrogel-based technologies to connect activity in cells reporting on behavioral experience with measures for both brain-wide wiring and molecular phenotype. We find that positive and negative-valence experiences in prefrontal cortex are represented by cell populations that differ in their causal impact on behavior, long-range wiring, and gene expression profiles, with the major discriminant being expression of the adaptation-linked gene NPAS4. These findings illuminate cellular logic of prefrontal cortex information processing and natural adaptive behavior and may point the way to cell-type-specific understanding and treatment of disease-associated states. |
| Databáze: | OpenAIRE |
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