Efficiency of modified skimmed milk base media to achieve high exopolysaccharide/cell ratios by Lactobacillus delbrueckii subsp. bulgaricus SZ2 in optimised conditions defined by the response surface methodology
| Autor: | Mahmoud Sheikh-Zeinoddin, Sabihe Soleimanian-Zad, Reihaneh Abdi |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
Sucrose food.ingredient biology Base (chemistry) Cell behaviour biology.organism_classification Industrial and Manufacturing Engineering Yeast chemistry.chemical_compound food chemistry Biochemistry Lactobacillus Skimmed milk Response surface methodology Food science Lactobacillus delbrueckii subsp. bulgaricus Food Science |
| Zdroj: | International Journal of Food Science & Technology. 47:768-775 |
| ISSN: | 0950-5423 |
| DOI: | 10.1111/j.1365-2621.2011.02906.x |
| Popis: | Summary Exopolysaccharide (EPS) production by Lactobacillus delbrueckii subsp. bulgaricus SZ2 was optimised in modified MRS (M-MRS) using the response surface methodology (RSM). Maximum EPS production was 74.3 ± 2 mg/L, and the optimised values of the three variables predicted for maximum EPS production included a temperature of 38.7 °C, Bacto-casitone and glucose concentrations of 24.5 and 29.6 g/L, respectively. To compare EPS production in MRS and skimmed milk (SM), the kinetics of EPS formation and growth were monitored in M-MRS, SM, skimmed milk plus 2% additional sucrose (Suc-SM) and skimmed milk containing Bacto-casitone (20 g/L) and yeast nitrogen base (5 g/L) (BY-SM). EPS production in all the media tested seemed to be growth-related. The EPS/cell ratios were determined to be 3.12 × 10−10, 1.43 × 10−10, 4.42 × 10−11 and 3.16 × 10−11 mg/cell, in Suc-SM, SM, M-MRS and BY-SM, respectively, clearly indicating the greater effect of C/N ratio when cell behaviour in EPS production is considered. |
| Databáze: | OpenAIRE |
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