Ontogeny and Biosynthesis of Isovitexin 7-O-Galactoside in a Mutant of Silene pratensis Unable to Glycosylate this Compound in the Petals

Autor:	J. van Brederode, J. M. Steyns
Rok vydání:	1983
Předmět:	Silene Ontogeny Isovitexin Mutant Caryophyllaceae Biology biology.organism_classification Galactoside General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound chemistry Biosynthesis Biochemistry Botany Petal
Zdroj:	Zeitschrift für Naturforschung C. 38:549-550
ISSN:	1865-7125 0939-5075
DOI:	10.1515/znc-1983-7-809
Popis:	Enzyme activity responsible for the biosynthesis of isovitexin-7-O-galactoside from isovitexin and UDP-galactose can be demonstrated in protein extracts of cotyledons and rosette leaves from Silene pratensis plants. This galactosyltransferase activity is absent in stem leaves and petals. “True Km” values of 0.0025 mᴍ for isovitexin and of 8.2 mᴍ for UDP-galactose were found. At infinite concentration of both substrates the “true Vmax” value was 0.11 mᴍ/min/mg protein. The galactosyltransferase activity was optimal at pH 7.0; the divalent ions Zn and Hg were inhibitory, whereas Ca, Co, Mg, Mn and EDTA were not inhibitory.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::76c974e6a0038153f3d8e49cae148fae https://doi.org/10.1515/znc-1983-7-809 Zobrazit plný text záznamu