| Autor: | Gerd Brunner, Volker Kasche, A. Overmeyer, Boris Galunsky, A.C. Chikere |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Aqueous solution
Chromatography Supercritical carbon dioxide Immobilized enzyme biology Aqueous medium Chemistry Soybean lipoxygenase 1 Bioengineering General Medicine Applied Microbiology and Biotechnology Lipoxygenase chemistry.chemical_compound Yield (chemistry) Carbon dioxide biology.protein Biotechnology |
| Zdroj: | Biotechnology Letters. 22:1815-1821 |
| ISSN: | 0141-5492 |
| DOI: | 10.1023/a:1005626806321 |
| Popis: | The activity of immobilised soybean lipoxygenase-1 (LOX-1) was studied in aqueous and supercritical carbon dioxide (SCCO2) media for the production of 13S-hydroperoxyoctadecadenoic acid (13S-HPODE). In SCCO2, it was optimal at 33 °C and 25 MPa. A higher space-time yield of 5.7×10−3 Ms−1 mg−1 LOX-1 for 13S-HPODE was obtained in SCCO2 compared to only 5×10−5 Ms−1 mg−1 LOX-1 in aqueous medium. The stability of immobilised LOX-1 was only significantly affected by the pressurisation and depressurisation steps during reactions in SCCO2. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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